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EmGFP was excited with the 488 nm wavelength argon laser line and confocal images were taken at a 500 570 nm emission setting using Leica TCS-SP software.
Fura-2 fluorescence was measured at wideband emission setting of 510 nm at 5 sec intervals for 10 min of baseline recording and then 10 min after exposure to drugs at excitation settings of 340 and 380 nm.
Upon proteolytic cleavage of the substrates by the different caspases, the free fluorochrome AFC was detected by a spectrofluorometer multiplate reader (FL-500; Bio-Tek Instruments, Milan, Italy) with excitation and emission setting of 395±20 and 530±20 nm respectively.
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By calculating the generating cost and carbon emission, set of non-dominated solutions is obtained.
Figure 1 Schematic diagram of the field emission set up housed inside the vacuum chamber.
Excitation maximum was determined for emission set at 516 nm.
To count motif occurrences, both emission set and value set are the natural numbers (or a finite subset thereof).
The excitation wavelength was set at 280 nm with the bandwidth of both excitation and emission set at 2 nm.
Fluorescence images were obtained by alternate excitation at 340 nm and 380 nm with emission set at 510 nm.
The derivatized glutathione was detected by a Waters ACQUITY UPLC® fluorescence detector with the excitation set to 390 nm and the emission set to 480 nm.
The fluorescence intensity was measured immediately using a fluorometer (LS50B; Perkin-Elmer, Waltham, MA, USA), with excitation set at 495 nm and emission set at 520 nm.
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