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The electron emission length (about 1 nm) has been estimated.
With an assumption that the average electron density and temperature in an emission length x, the photon intensity I λul) of a spectral line can be written from the quasi-steady-state approximation of the CR model as[20] I ˜ λ ul = PE C ― recombining N ˜ e N ˜ i x + PE C ― excitation N ˜ e N ˜ g x, Open image in new window (3).
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The excitation and emission wave length used were 360 nm and 440 nm respectively.
The measurements were made with excitation wavelengths of 340 and 380 nm and an emission wave length of 505 nm.
The intact nuclei containing fragmented DNA show fluorescence at 510 nm emission wave length (TUNEL-positive nuclei).
The fluorometric analysis of the resultant product resorufin was done with an excitation at 530 nm and an emission wave length of 585 nm.
Total fluorescence of the cell population was measured using a microtitre plate reader (Synergy2, Bioteck) set up in the fluorescence mode with excitation wavelength set to 485 nm and emission wave length set to 530 nm.
The structures were inspected with a Leica TCS SP5 confocal microcroscope using the appropriate excitation and emission wave lengths.
All QD-bound proteins, except the negative control QD705-SLN, bound specifically to Importin α resin and could be distinguished on the same bead based on their corresponding QD emission wave lengths (Figure 1C).
Second, color-coding of proteins with QDs of different emission wave lengths offers the unique opportunity to monitor multiple binding events in a single experiment on a single bead.
For each measurement site the 2006 NO X emissions and length of road was taken from the LAEI.
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