Exact(3)
As can be seen, the silver stain does not readily show the capsule which is visible in the ProQ Emerald stain.
However, we were not able to detect it using Pro-Q Emerald stain (data not shown).
This is most likely due to glycosylation because immunoprecipitated Slp1-3xFLAG can be visualized by Pro-Q Emerald stain, which detects periodate-oxidized carbohydrates.
Similar(57)
ProQ Emerald staining detected six glycoprotein bands in P. arabicus salivary gland samples.
Carbohydrates were detected on immunoprecipitated proteins after SDS-PAGE using the Pro-Q Emerald staining kit from Life Technologies according to the manufacturer's instructions.
The lipoglycans were visualized using Pro-Q emerald glycoprotein stain (Life Technologies, Darmstadt, Germany).
The gels were then incubated in fresh Pro-Q Emerald 488 staining solution for 3 h in the dark.
After electrophoresis, the LPS was stained with Pro-Q Emerald 300 LPS stain kits (Invitrogen, Carlsbad, CA), according to the manufacturer's recommendation, visualized, and photographed using the AlphaImager digital imaging system (Alpha Innotech Corp., San Leandro, CA).
Gels were stained for total proteins with Coomassie G-250 (SimplyBlue SafeStain, Invitrogen) or for glycoproteins with Pro-Q Emerald 300 glycoprotein stain (Invitrogen).
Glycosylation of proteins on an SDS-PAGE gel was detected by using the Pro-Q Emerald 300 glycoprotein stain (Invitrogen) following the manufacture's instruction.
For the control assay, we used a carbohydrate staining kit (Pro-Q Emerald 300 Glycoprotein Gel Stain Kit, Molecular Probes; Figure 4(a), lane 1), and we observed a range of glycoproteins from 29 kDa to 116 kDa, with more intense bands of 29, 45, and 66 kDa.
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