Considering these many different roles, it is likely that the cholinergic system will not function in the same manner throughout embryonic development, taking over its main role within the nervous system only at later stages.
enaGC5 (from Bloomington, stock #8570) is caused by an inversion (breakpoints at 44E and 56B) [41]; it is embryonic lethal over enaGC1 [39] (own observations).
enaGC1 (from Bloomington, stock #8569) is a protein null allele due to a chromosomal inversion (breakpoints at 55B and 56B5) which causes severe axonal growth phenotypes [41]; it is embryonic lethal over ena23 [32] (own observations).
This enables a more continuous view of embryonic development over the first two days because the 48 hour samples from Mexico used here are the same as those used in Voolstra et al. [27].
While previous studies have not systematically characterized the viscoelastic properties of an embryonic tissue over a wide range of loads and loading rates, our results are consistent with previous studies on other embryonic tissues using tensile, compressive, and indentation tests [59], [60], [61], [62].
Epiboly represents the spreading of embryonic cells over a vegetal yolk mass, resulting in ectoderm to cover the embryo.
We therefore assayed tip growth by measuring the length of the embryonic axis over a three-day period.
The lethal phase suggested varying strength of the alleles, as cyclin E B29-25l, cyclin E 22t, and cyclin E 10.73 were embryonic lethal over the deficiency, whereas cyclin E and cyclin E 1f36 were larval lethal in trans to the deficiency.
Importantly in addition, as suggested by the reviewer, we further challenged our findings by now measuring embryonic viability over a wide range of temperatures upon depletion of key aerobic metabolism proteins (subunits of complex I, III and V of the mitochondrial respiratory chain).
His previous two one-day internationals had brought him no wickets for 122 from 15 overs and an embryonic question-mark over his temperament.
