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We previously developed a structured basement membrane mimic, a synthesized basement membrane (sBM) substratum, in which laminin-111, a unique component of embryonic lungs, is incorporated.
FINDINGS: Analyzing the development of embryonic lungs from chicken, quail, and duck, as well as lungs explanted and cultured ex vivo, revealed that the patterns of branching are remarkably conserved.
Embryonic lungs (Fgf10 +/- and Fgf10 +/+ [WT]) were harvested at E12.5 and E18.5.
Paraffin sections of embryonic lungs were hybridized using a protocol adapted from [45].
The mothers were injected with supraphysiologic doses of IL-4 or IL-13, and the nuclear extracts of the embryonic lungs were analyzed using Western Blot for presence of phosphorylated STAT-6.
All experiments were performed with mouse embryonic lungs.
Similar(27)
Then, one strain of the H9N2 subtypes was proliferated in the embryonated chicken eggs (ECE) and human embryonic lung fibroblast cells (MRC-5) system.
Waghray A, Rajagopal J. Tips from the embryonic lung.
Bovine embryonic lung fibroblasts from 99-day fetuses were generated by Dr. Neil Talbot in 2009.
Wnt7b stimulates embryonic lung growth by coordinately increasing the replication of epithelium and mesenchyme.
TE11 p39 Bovine embryonic lung fibroblasts were obtained from Dr. Toshihiko Ezashi and Dr. R. Michael Roberts of the University of Missouri-Columbia.
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