Additionaly, zMELK and human MELK genes are preferentially expressed in embryonic brains and retinas, especially in the proliferative ventricular zones, where neural stem cells are localized.
As an example, circular RNA molecules derived from Sry gene are the main transcripts in embryonic brains on Days 11 19, whereas linear molecules become more ample in postnatal brains, wherein circRNAs can even be absent (Mayer et al., 2000).
The growth of both small-sized and large-sized NSs from embryonic brains were significantly accelerated by the HMF-exposure (Figs. 1, 2 and 7), indicating a common pro-proliferation effect on both NPCs and NSCs.
PND0 brains were processed as described for embryonic brains.
Fixed embryonic brains were then cryoprotected overnight at 4°C in 30% sucrose in PBS.
Cryoprotected embryonic brains were embedded in Tissue-Tek O.C.T. compound (Sakura) and frozen in 2-methylbutane chilled with dry ice.
We previously generated a specific siRNA against Ndel1 to knockdown the protein in primary neurons and mouse embryonic brains [18], [19].
The STIM1 antibodies we used did not stain neurons in the sections of the STIM1 knockout embryonic brains obtained from Nieswandt's group.
