However, even a mild reduction in the expression of these proteins resulted in arrest during early embryonic development, precluding assessment of genetic suppression (data not shown).
Assessment of embryonic quality requests noninvasive or minimally invasive techniques that do not interfere with embryonic development to term.
A dynamic assessment of embryonic development (cleavage kinetics) using automated time-lapse imaging systems may have the potential to improve oocytes/embryo selection [ 3].
Dynamic assessment of embryonic development by time-lapse imaging based on morphological grading as well as providing kinetic parameter presents another opportunity for optimizing embryo selection.
Unfortunately, in both models, the significant amount of embryonic lethality hindered a comprehensive assessment of the role of IKKβ in the formation and function of other vascular beds at later stages of development.
This technique was previously employed by our team and other colleagues to source embryonic DNA for preimplantation genetic assessment, avoiding invasive biopsy procedures (Perloe et al, 2013; Poli et al, 2013; Gianaroli et al, 2014).
To meet the scientific objective of prospective assessment of periconception, embryonic, and early fetal exposure, the initial vanguard cohort of the NCS was designed to enroll women as early in pregnancy as possible, and also to enroll some women who were not currently pregnant, but heterosexually active and at high probability of becoming pregnant in the near future [ 8].
The assessment of cleavage rate and embryonic development in vitro confirmed that the designed 4-step adding 2-step diluting protocol indicated a better outcome.
These studies also suggest that the embryonic zebrafish model is well-suited for the rapid assessment of nanomaterial toxicity.
The storage, management and use of mobility data either generated by autonomous cars, by V2I sensors or by smartphone apps are still at an embryonic stage and initiatives such as the Privacy Impact Assessment [79] should be used more widely at European or international levels to identify and address such concerns.
