Sentence examples for embryo solution from inspiring English sources

Injected and control noninjected embryos were immersed in 1× Yamamoto embryo solution with 0.1 μl/ml of Methylene Blue and incubated at 26° until they developed eyes (6 8 days).

At 10 hpf, embryos were de-chorionated with forceps and transferred to a glass vial with 3 ml Embryo Solution, and Selamectin was added into solution to a final concentration of 2 μM.

Eggs were sieved from the sand with a strainer and kept at 4° in 1X Yamamoto embryo solution (17 mM NaCl, 2.7 mM KCl, 2.5 mM CaCl2, 0.02 mM NaHCO3, pH 7.3) (Westerfield 2000) with 0.1 μl/ml of Methylene Blue (Kordon, 2.3% stock solution) to limit parasitic infection.

Lassalle et al. (1985) froze embryos in solutions with or without 0.1 M sucrose, but had insufficient evidence to comment on the value of adding sucrose.

After incubation in blocking solution, embryos were transferred to a solution with 3G8 and/or 4A6 monoclonal antibody [42] followed by incubation with anti-mouse alkaline phosphatase IgG (Sigma) and developed using SIGMAFAST fast red tablets (Sigma).

RNA was extracted from WT or from mutant embryos using Trizol solution according to the manufacturer's instructions (Invitrogen).

Staining for erythrocytes was achieved by incubating embryos in a solution containing o-dianizidine (Sigma, St Louis, MO) as described (Paffet-Lugassy and Zon, 2004).

Induction of enterocolitis by DSS exposure was carried out essentially as described for TNBS (Oehlers et al., 2011a), with the substitution of DSS, or dextran as a control (MP Biomedicals), dissolved in E3 embryo medium for solutions of TNBS.

After repeated washes with the M9 isotonic solution, embryos were maintained in a shaker in 3 to 5 mL M9 in 15 mL conical tubes, at 20°C and 120 rpm.

Embryos were dechorionated and placed in 1/1000 dilution of the acridine orange stock solution in embryo medium for 30 min. After washing in embryo medium, the embryos were placed into tricaine anesthetic solution (3-amino benzoic acid ethyl ester; Sigma-Aldrich) (70) and viewed immediately using a green fluorescence filter.

As recommended by the manufacturer, the embryo was washed in Solution 1 at 37°C for 30 s and then moved into Solution 2 at 37°C for 2 min.