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Human embryo kidney cell line HEK293T/17 was originally purchased from ATCC (No. CRL 1573) and maintained in Dulbecco's Modified Eagle's medium (DMEM, Gibco, BRL).
Human cervical carcinoma cell line Hela, human embryo kidney cell line 293, murine melanoma cell line B16-F10, and murine breast carcinoma cell line 4T1 were obtained from the ATCC (Manassas, VA, USA).
The HEK293 ZsGreen Proteasome Sensor Cell Line (HEK293PS), a stably transfected human embryo kidney cell line expressing the ZsProSensor-1 fusion protein, was obtained from Takara Bio (Tokyo, Japan).
BEL7402, SMMC-7721, HHCC2, HHCC and Chang liver (transformed liver cell line) were preserved in our institute and HEK293 (Ad5 E1-transformed human embryo kidney cell line) cells were purchased from American Type Culture Collection (Manassas, VA).
The adenovirus-transformed, human embryo kidney cell line 293T [ 23] and the rat glioma cell line C6 (ATCC CCL-107) were maintained in DMEM (Invitrogen, USA) supplemented with 10% bovine calf serum (BCS, HyClone, Logan, UT, USA), 100 μg/ml gentamycin, 50 μg/ml ampicillin, and 2.5 μg/ml fungizone, at 37 °C, in a humidified atmosphere of 5% CO2.
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Mouse myoblasts (C2C12), human embryo kidney cells (HEK293), and human umbilical vein endothelial cell lines (ECV304 and EA.hy926) were cultured with high glucose DMEM.
Some types of cells, such as HEK293 (human embryo kidney cells), tended to grow in clusters on the hydrogel surface (Supplementary Fig. S3), suggesting that the sericin hydrogels can influence the spatial distribution of cells.
Green fluorescent protein that had been inserted into human embryo kidney cells was used in a tiny optical cavity to produce laser light.
The effects of the minor G and major A alleles of rs2243890 on the promoter activity of RCAN1.4 were separately analyzed in the human embryo kidney cells (293T), the rat cardiomyocyte-derived cells (H9C2) and the mouse myoblasts (C2C12) using dual-reporter luciferase system.
Porcine embryo kidney cells (PS) were used to produce TBEV stocks, to recover mutant viruses, for plaque assay and studies of cytopathogenicity.
All strains were passed 3 times in chicken embryo kidney cells.
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