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10 Maternal insulin resistance can negatively impact the developing embryo due to impaired glucose transport.
In particular, we suggest that you could consider removing parts from the early embryo due to lack of functional relevance.
Secondly, the internal distribution of the molecules in the embryo due to the physicochemical nature of the molecules can be examined and compared at different stages of development.
The effector constructs are uniformly distributed in the entire embryo due to the presence of the SB direct-inverted repeats [ 15].
In the early embryo, due to their different expression patterns and their distinct additional roles of promoting Sog function (αPS1) or providing positive feedback on BMP signaling (αPS3), we expect αPS1 and αPS3 to act only partially redundantly.
Histological observations and immuno-staining have not allowed direct identification of the hemangioblast in the early embryo due to the rarity of these cells and the lack of known uniquely specific markers; and experimental evidence for the hemangioblast in vivo has been obtained only relatively recently using in vitro assays [ 4- 7].
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In practice the cells come from excess frozen embryos due to be discarded by fertility clinics.
The rules would permit federally financed researchers to use cell lines that were derived from frozen human embryos due to be discarded by fertility clinics, usually because the owners no longer wanted them.
To estimate cumulative chance for first live birth after elective pronuclear stage cryopreservation of all embryos due to ovarian hyperresponsiveness.
The effect of dechorionation was demonstrated with the cationic polymer Luviquat HM 552, which is blocked by the chorion non-dechorionated embryos due to its molecular weight of ~ 400,000 Dalton, but becomes strongly toxic after dechorionation.
In conclusion, we inferred that the washing protocol of 10 washes and two trypsin treatments did not eliminate EAV from all embryos; due to limitations in experimental design, this requires confirmation.
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