Fixation and embedding of cardiac tissue samples were performed as described earlier.
To monitor the distribution of chemicals and drugs, the precision was greatly improved by the introduction of precise cryosection(1612) of freeze-dried samples, 1613) which avoided fixation and embedding of the samples.
Freeze substitution and resin embedding of the samples were compatible for sectioning at room temperature and classical EM treatment.
RNA to quantitate both IL-8 mRNA, VEGF mRNAs and miR-93 was extracted from formalin-free alcoholic-based fixative FineFIX and paraffin embedded samples of the archive of deceased patients by MiRNeasy FFPE minikit (Qiagen, Venlo, Limburg, Netherlands).
Samples used for this study were collected from the depository of formalin fixed paraffin embedded samples of Sikl's Department of Pathology, Medical School and Teaching Hospital in Pilsen.
Macroarrays were prepared by joining and re-embedding of four to nine tissue samples in novel FFPE blocks.
When the molecular structure of the resin is high enough to not penetrate the samples' pore system, the technique of embedding the sample in a hardening resin on a hardened bed can be used.
In addition, synthetic sequence of the same length, but containing embedded samples of PWMs rather than actual REDfly footprints, was included.
The hydrogels look aggregated and fused which we believe is due to the polymerization temperature (≥60 °C) during resin embedding of the sample for imaging.
