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Adding a few flourishes of his own to its appliqued trompe-l'oeil walls and ceiling, he'll include an embedded slide show and double-sided sketches by Eyre de Lanux, a expatriate painter, writer and Art Deco furniture designer known for her liaison with the writer and saloniste Nathalie Barney.
100 µl of this culture was plated onto the Petri dish containing the embedded slide and allowed to dry for 30 min. The slide was removed from the Petri dish, a cover slip placed on top and the excess solid medium trimmed from around the edges of the cover slip.
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I've embedded slides from the presentation below.
Paraffin embedded slides were then treated as per instructions in the apoptosis detection kit.
RNA was isolated from formalin fixed paraffin embedded slides using the RecoverAll RNA kit (Ambion, Cat #AM1975).
Portions of each tumor were prepared as formalin-fixed paraffin embedded slides and soluble cell lysates (excluding sample NL2 and NL8 due to small tumor volumes).
In brief, paraffin embedded slides were treated with hydrogen peroxide (H2O2) to block endogenous peroxidase activity, and washed with ddH2O and PBS.
Formaldehyde and paraffin embedded slides were examined by pathologist who determined the type of cancer (or lack of it), tumor grade and Gleason sum.
Paraffin embedded slides were labeled with anti-CD44 (Cell Signaling, Danvers, MA) and anti-c-Met antibodies (Cell Signaling) and stained as previously described [ 11].
Sections from samples for which we had fixed paraffin embedded slides were tested for pERK (Tables 1, 2a and b; Figure 3) immunoreactivity and scored as previously described (Faury et al, 2007).
These came in two separate sets: 20 fresh frozen tumour blocks with FFPE slides from the same tumours (ER-positive = 10; ER-negative = 10) and 135 tumours that were provided as formalin-fixed paraffin embedded slides (FFPE; n = 45 ER-neg, n = 45 ER-positive grade 2, n = 45 ER-positive grade 3; 10 slides from each tumour, 3-μm sections).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com