Although the group with sensory nerve tracts showed a little weaker effect compared to the vascular group, embedding nerve tracts into the tissue engineered bone could still improve the neurotization and osteogenesis of the bone graft compared to those in blank group.
After epoxy resin embedding, nerves were cut transversally (0.3 μm thick sections), stained with 1% toluidine blue solution, and observed under the oil immersion lens of a light microscope.
For neuromas, we recommend using sharp dissection and nerve end ligation, followed by embedding the nerve end with epineurium.
The surgical resection of a neuroma was achieved by sharp dissection and nerve end ligation, followed by embedding the nerve end with epineurium.
The normal myocardium is composed of cardiac cells, blood vessels and nerves embedded within a complex three-dimensional space, the interstitium or extracellular space.
EFS with short pulse width (0.1 ms) generated contractions that were blocked by the neurotoxin TTX (1 μM), suggesting that these contractions were mediated by stimulation of the nerves embedded in the bladder wall.
The ability of the peptidergic nerve fiber to regulate the function of osteoblasts and blood vessels can be used to construct the neurotized tissue engineered bone by embedding the nerve fiber into the center of bone graft [ 19– 219.
Paraffin-embedded nerves were sectioned at a thickness of 6 μm for the entire length of the nerve, including the site of injection, and mounted on glass slides.
The resin-embedded nerves were cut with an Ultracut UCT ultramicrotome (Leica Microsystems, Wetzlar, Germany) with a thickness of 2.5 μm starting from the distal end of the median nerve and stained with 1% Toluidine blue in 1% borax for 30 45 sec while on a hot plate.
Different subsets of nociceptors respond to different combinations of stimuli, depending on which protein sensors are embedded in their nerve terminal membrane, and animal venoms act on these proteins in some way or another.
