To overcome this difficulty, the Flash file was embedded in a Flash Projector (Zinc 3 produced by MDM) that turns Flash files into executable files.
For SA- βgal staining in tissues, 21 days following tumor inoculation, mice were euthanized and tumors collected, embedded in OCT, flash frozen in liquid nitrogen and stored at −80 °C.
Hearts were excised, cut in the coronal plane into three sections from apex to base, embedded in OCT, flash-frozen in liquid nitrogen, and stored at −80°C.
In the new study, the researchers set out to adapt the expansion process for biopsy tissue samples, which are usually embedded in paraffin wax, flash frozen, or stained with a chemical that makes cellular structures more visible.
The bladders were excised, embedded in OCT medium, flash frozen in liquid nitrogen and sectioned.
Each biopsy specimen was immediately embedded in OCT and flash frozen in isopentane immersed in liquid nitrogen.
For all immunofluorescence staining, skin samples were directly embedded in O.C.T. compound, flash frozen, and stored at -80°C.
Tissues were then embedded in OCT compound, flash frozen, and stored at –20°C for subsequent sectioning (10 μm) in a cryostat.
These products are masks that have led lights embedded in them that flash near the eyes if you wear the mask while sleeping.
Blinking lights embedded in the street also flash when a pedestrian pushes a button to cross the road.
