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The medium either was directly poured to Petri dishes for standard spreading or streaking, or was mixed with cell cultures prior to pouring for embedded growth.
Considering that Thermotoga can tolerate brief exposures to oxygen, we simplified the overlay methods used by other groups (Kenneth Noll, University of Connecticut, private communication; [ 16]) and developed an embedded growth method.
This positioning is likely true for cells growing within a homogenous environment, such as in liquid media or during embedded growth in agar, but an asymmetrical, or 'nose-down', tip morphology has been observed in several fungi during growth on surfaces (Read et al., 1992; Bowen et al., 2007).
As illustrated in Figure 8D, this chimera localized to the plasma membrane, yet was still unable to complement the C. albicans embedded filamentous growth defect of a rac1 deletion strain, making it unlikely that inefficient membrane targeting can explain HsRac1 inability to function in embedded growth.
"There's a lot of embedded growth in Tom Brown's assets," Mr. Deacon said, projecting an annual double-digit increase in the American company's output over the next seven to eight years.
A practical IRT model with an embedded growth model is illustrated to examine the psychometric characteristics of classroom assessments for preschool children, and also to show how nonlinear learning over time can be investigated.
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Similarly, Rac1CTCdc42 was functional, when expressed as a sole Rac1 copy, for embedded filamentous growth, suggesting that Rac1 nuclear accumulation is not required for embedded filamentous growth (Figure 3C).
He expected China Life to be hard pressed to achieve more than 10% embedded value growth year-on-year for the first half of 2008.
As expected if Rac1 functions primarily at the plasma membrane, rac1-5Q and rac1[C233S] were unable to restore embedded filamentous growth in a rac1 deletion mutant (Figure 4D).
The rings were embedded in growth factor-depleted Matrigel and examined for endothelial sprouting upon simulation with FGF-2 (50 ng/ml).
To promote resident stem cell function, microbeads embedded with growth factor can be transplanted in the infarcted area.
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