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The temperature, relative humidity, and barometric pressure are measured and the data are saved to external EEPROM for later extraction.
The biopsies were immediately frozen and stored in liquid nitrogen for later extraction of RNA.
Cells were trypsinized, cell suspensions combined and centrifuged and pellets stored at -80° for later extraction.
Each synovial specimen was divided into two parts: one was formalin fixed and paraffin embedded for immunohistologic analysis and the second was stored in RNA later (AMBION Life Technologies Ltd ,UK) at −80°C until RNA extraction and quantitative PCR analysis.
Immediately after collection, tissue was placed in RNAlater (Applied Biosystems, Warrington, UK) for RNA extraction, fixed in 4% neutral-buffered formalin, and wax embedded for immunohistochemistry (IHC).
A piece of each was stored at − 70 °C for later RNA extraction and another piece fixed with formalin and embedded in paraffin for histological analysis.
Table 9 Cost of computation for embedding and extraction Methods Embedding Extraction Time in sec Time in sec DWT SVD 0.269155 0.60672 RDWT SVD 1.192001 2.045824 Proposed method 0.99354 0.993546.
All YoYs captured were euthanized and stored whole in 95% ethanol for later DNA extraction.
These were stored in liquid nitrogen for later RNA extraction.
Tumors were immediately flash frozen with liquid nitrogen for later protein extraction.
The DL supernatant was immediately divided into aliquots and stored at -80°C for later DNA extraction.
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