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For histology, 5 µm thick sections were cut from formalin fixed, paraffin embedded, blocks and stained with hematoxylin/eosin.
Dehydrated samples were routinely processed into paraffin embedded blocks.
Speed of processing the sample into wax embedded blocks also may influence immunostaining.
Five-micron sections were prepared from paraffin embedded blocks on poly-l-lysine coated glass slides.
This was done with immunohistochemical methods coupled with a morphometrical assessment using the paraffin embedded blocks of the previous study.
The method facilitates easier sample handling and relocation of cells in embedded blocks.
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Human samples were processed directly from paraffin-embedded blocks.
Immunohistochemical staining was performed on 5 µm thick sections prepared from formalin fixed, paraffin-embedded blocks.
TMA obtained from paraffin-embedded blocks was used for the immunohistochemistry experiments.
Tissue sections 4 µm thick were cut from paraffin-embedded blocks and placed on glass slides.
Formalin-fixed, paraffin-embedded blocks were processed as described [23] using heat antigen retrieval and avidin-biotin complex detection.
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