The observed dot-staining pattern for Plk4 contrasts with previous immunofluorescence and immuno-EM data from our laboratory, showing a ring-like disposition of Plk4 around the circumference of the mother centriole (Habedanck et al., 2005; Kleylein-Sohn et al., 2007).
I. L. collected the EM data with help from A.W. I.L. performed data processing and refinement with help from M.A.C. I.L. and F.D. generated the atomic models with homology models generated by J.X., W.W. and D.W. D.W. and A.E.L. wrote the manuscript with help from all laboratory members.
The pause-free elongation rates agree with data obtained from EM data (Pollard, JCB, 1986) (misnamed "bulk" in the manuscript).
It is also clear from our EM data that autophagosomes (exemplified by a double membrane) are present in cKO RPE, where they accumulate, due to inhibition of the proteolytic degradation by the lysosomes (Fig. 3C and D, arrows).
We did state that MT shape and protofilament number appeared grossly wild-type, but with so few MTs examined, perhaps we should not have attempted to make any statement regarding the VNC MTs from our EM data.
Electrophoretic mobility (EM) of the colloids and sand fragments (Supporting Information S6) suspended in DI water, the CaCl2 solution, and flowback fluid was measured using the above zetasizer, and zeta potentials were calculated from the EM data by Smoluchowski's equation.
Dephoure N, Hwang S, O'Sullivan C, Dodgson SE, Gygi SP, Amon A, Torres EM, 2014, Data from: Quantitative Proteomic Analysis Reveals Posttranslational Responses to Aneuploidy in Yeast, http://dx.doi.org/10.5061/dryad.65364, Available at Dryad Digital Repository under a CC0 Public Domain Dedication.
EM-clustered data from C3 C4 intermediate species were used to generate posterior probability distributions for the timing of the acquisition of C4 traits in sixteen evolutionary steps (A ) or four quartiles (B ).
The cryo-EM data were collected from the Electron Microscopy Laboratory and Cryo-EM Platform at Peking University.
