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Yet, the extremely small subcellular volumes represented by EM images and potential artefacts from physicochemical processing of membranes clearly limit stand-alone insight from EM analysis and require balancing of approaches through independent methods.
Recently, graphene attracted a great deal of attention of EM analysis and characterization [7, 8].
Only a minority of enterocytes might display the hallmarks at the time of EM analysis, and the histological presentation of MVID varies from patient to patient.
We thank Alice Liang for EM analysis and Peter Lopez for cell sorting.
We are grateful to Dr J. Tovar for the gift of the antibody against IscS, Dr. P. Wild for cell preparation for EM analysis, and T. Michel for excellent technical assistance.
In order to identify candidate RER whorl forming proteins in Ae aegypti midgut epithelial cells, we took advantage of the fact that an amino acid meal is sufficient to induce whorl unwinding, which simplifies both EM analysis and protein identification by mass spectrometry because it eliminates interference from abundant blood meal proteins.
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First, cryo-EM analysis and lysozyme treatment clearly showed that the spinae are not extensions of the cell wall, hence belonging to the family of non-prosthecate appendages.
Supporting information describing cryo-EM analysis and 3D reconstruction of the N255K samples are shown in Figure 7 figure supplement 1. DOI: http://dx.doi.org/10.7554/eLife.04686.024 10.7554/eLiFigure86.025 Figure 7 figure supplement 1. Analysis of cryo-EM images of N255K kinesin complexed with microtubules (see Figure 7 ).
SvL, BT, AvD, PW and AW contributed significantly to the discussion, KV and SP performed immunostainings and sequence analyses, IO performed EM analysis, RAW and AR were responsible for LC-MS analysis and WT and CJFvN performed the enzymatic mapping assays.
Considerable electromagnetic (EM) analysis efforts and top-down CAD approach enabled the design of a final and consistent concept, starting from a very first dimensioning for EM loads.
Moreover, our EM analysis [13] and TUNEL staining (Figure 2) did not detect apoptosis in the Aβ42 fly brain.
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