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The resulting species eluted as a single peak at elution volume 50 mL, which is consistent with the formation of a protein complex that is larger than ASB9 EloBC (elution volume 57 mL) or Cul5NTD (elution volume 54 mL).
Elution volume was compared against the elution volume of standards (GE Healthcare) to determine molecular weight.
At higher concentrations, a small oligomer was observed at an elution volume of ~61 ml.
When all wells are totally dry, add 75µL (or other elution volume) of ddH2O to wells.
NOTE: 75µL elution volume will give you a concentration range from 300-800ng/µL 300-800ng/µL
Secondary PCR products were cleaned and then size selected as described above with a final elution volume of 30 μl.
Bigger oligomers with an elution volume of <~57 ml were also detected at a higher concentration of 7 mg/ml.
However, for mixtures of PDLA with PLLA an additional peak was observed at higher elution volume.
The elution volume of the smaller oligomers was compared to those of the cross-linked TTR tetramers and octamers (Fig. S2).
Two 0.5 ml fractions were collected around an elution volume of 11.5 ml, where the peak had the highest intensity, and the sample was concentrated.
The association of Erh1 with Mmi195 122 resulted in a shift of about 0.25 mL in elution volume to ~11.16 mL, with a calculated MW of 33 kDa.
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