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Since the latter three compounds elute after furfural their elution times are in the range of 1 2 h on the 300 mm column (data not shown).
Most of the αB-crystallins eluted from the size exclusion column as relatively broad symmetrical peaks at early elution times indicating they exist as polydisperse high molecular mass oligomers (Fig. 1C).
(See text for peak elution times).
Subunit elution times and integrated areas were highly repeatable even after several hundred injections.
A selectivity test anticipates to obtain a chromatogram having specific elution times for all known compounds.
However, increasing elution times, as per these data, does not compromise the structural integrity significantly.
Tests were conducted to study changes in hardness of AIBC following increased elution times and antibiotic loads.
Separation of CVA and IS was achieved using optimum HPLC conditions with elution times of 2.780 and 1.293, respectively.
This study on hardness vs. elution times and increased antibiotic dosages demonstrates the increased susceptibility to these problems.
At intermediate reaction rates, the elution profiles are stretched between the characteristic elution times of the two components.
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These elution times were expected for non-derivatized amino acids on the reversed-phase column.
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