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Before self-assembly into micelles, the unimers were eluted at higher elution time (12.5 min) indicating hydrophobic character.
Using this formula, 76.7% of 8-plex peptides detected with the stringent criteria eluted within a time window of ±3 min of the predicted elution time.
A global optimum was obtained by selecting the elution time, the sensitivity and the overall resolution as responses to optimize.
The initial bolus release was characterized by predicted pin tract gentamicin concentrations of >80 μg/ml at the 2 h and 1 day elution time points.
Also, the optimal values for the elution step were 6.5 mL of HCl (4 M) as the eluent volume and type and elution time of 120 min.
Elution time of intact complex is indicated by grey box.
Dashed lines mark peptide elution time predicted by iRT-calibration.
This, however, does not change with increasing elution time.
Mass spectra of betulin (M=442, elution time 23.7 min) and lupeol (M=426, elution time 19.07 min) are displayed in the Figures 8 and 9, respectively.
Increased elution time, after 1 day, however, does not seem to have the same effect.
Figure 9 The chromatogram and mass spectrum of lupeol, (ethanol/methanol, elution time 19.07 min).
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