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The elution schedule is presented in Table 1.
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It includes the adjustments of the fill time of the trapping device and the tight scheduling of elution times of the peptides, ideally adjusted on-the-fly.
DNA was eluted with an elution buffer.
Purified DNA was eluted in 80 μl elution buffer.
Aside from polar pesticides, other pesticides were well distributed across the elution window facilitating proper scan rate for scheduled MRM methods of targeted analytes as shown in Fig. 3.
The plasmids were eluted with 30 μl of elution buffer.
After isolation, the purified DNA was eluted in 100 μL of elution buffer.
The purified DNA was eluted in 200 μl of elution buffer and stored at −20 °C.
Elution was done with 1 1 methanol/isopropanol, after which the eluted sample was dried.
DNA was eluted by three successive elutions each with 100 μl DNA elution buffer and stored in sterile microtubes at −20 °C.
Elution was analyzed by mass spectrometry.
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