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Dichloromethane/methanol (1 1) was used as a mobile phase to elute the resins at an elution rate of 1.5 cm3/s.
The parameters are in vitro VAN elution rate, in vitro activity of the eluted VAN against planktonic Staphylococcus aureus (S. aureus), a microorganism commonly found in PJI cases [ 24], and activity of eluted VAN against S. aureus inoculated in a periprosthetic rabbit model.
In in vitro tests, we found that when VAN- (VAN-) loaded PMMA bone cement specimens subjected to MB-mediated US, both the VAN elution rate and the bactericidal effect of the eluted antibiotic against planktonic S. aureus were each significantly greater compared to cases when either VAN alone or VAN + US was used.
Model predictions that tissue content linearly tracks stent elution rate were validated in porcine coronary artery sirolimus-eluting stents implants.
Peptides containing photo-cross-linker (benzophenone) were eluted with gradient 15 40% buffer B in 40 min; all other peptides were eluted with gradient 5 35% buffer B in 40 min. The elution rate was 15 mL/min.
Ligand density has a less pronounced effect on the elution rate, presumably due to ionic strength-dependent changes in the pore architecture of the sorbents.
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The effects of geosorbants on effective elution rates were compound- and sorbent-specific.
Iron and iron-bearing clays reduced the effective elution rates of both RDX and TNT.
An automated Accelerated Solvent Extractor was used to determine the effective elution rates under controlled conditions of RDX and TNT from soil columns containing particles of Comp B. Contrived soils containing selected soil geosorbants and reactive surfaces were used to quantitatively determine the importance of these materials.
Elution rates ≥10ml/min result in RCP values of <95%.
If dextran or glycine type space fillers, which have previously been proven to increase elution rates [12, 21].
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