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Forward sensitivity analysis is more straightforward to apply, as no perturbation factor needs to be chosen, and even computes faster than finite differences for a benchmark example with three chemical components and load, wash and elution phases.
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2 mL fractions were collected during the elution phase.
During the elution phase current protocols are likely to cause CD34+ cells to exceed tolerable limits.
Two solvents were used for elution: Phase A (0.05% trifluoroacetic acid in water) and Phase B (0.05% trifluoroacetic acid in acetonitrile).
Tests were conducted only on the surface of the cement, indicating that during the burst elution phase, the surface hardness of AIBC deteriorates, thus weakening the structural integrity of the cement.
The extracts were re-dissolved in 2.0 mL elution phase, prior to HPLC analyses for ester-linked phenolics.
The excess ligands and free ions were removed by passing the sample through a Sephadex-20 column with PBS as elution phase.
For ZEN14Glc and ZEN16Glc, a short isocratic elution phase between 7 and 9 min had to be introduced during the linear gradient programme to achieve chromatographic separation.
The extracts were re-dissolved in 2.0 mL elution phase, then it was filtered by 0.22 μm membrane and used for HPLC analyses.
A DNAse I treatment was performed directly through the column used to collect RNAs and before the elution phase of DNA-free RNA.
For C-His6-tagged VanYn, 6 M urea was added to the equilibration buffer as denaturant; in order to refold the protein bound to the column, a linear gradient from 6 to 0 M of urea (2 mL/min) was performed before starting the elution phase [ 29].
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