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Our findings show that the hitherto elusive sites for the formation of the Tyr intermediates in CcP are Tyr71 and Tyr236, and given the recent report of guaiacol having a binding site close to Tyr71, we investigated its putative role in guaiacol oxidation.
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However the details of the chemical mechanisms controlling selectivity and activity have remained elusive because important sites in these structures are occupied by mixtures of Mo and V atoms, obscuring the actual distributions of the metals and oxides at the active sites.
The strategy used to identify the elusive Tyr radical sites in CcP may be applied to other heme enzymes containing a large number of Tyr and Trp residues and for which Tyr (or Trp) radicals have been proposed to be involved in their peroxidase or peroxidase-like reaction.
Evidence suggests that this is the first direct visualization of the elusive inhibitory metal site, whose existence has hitherto only been inferred from kinetic data.
A comparative analysis of East African agroecosystems shows that thresholds in specific variables that may point to the existence of possible tipping points are rather elusive and largely site specific, requiring systematic categorisation of agricultural contexts.
The large number of elusive or weakly linked sites will also elude Web crawlers, meaning that surfers who rely on search engines "probably won't be able to reach substantial portions of the Web," said Andrei Broder, a vice president for research at the AltaVista Company and a member of the research group.
Doping engineering is widely utilized to tailor the electrical property, while investigation on the dopant-induced modification of active sites remains elusive.
Although there is a vast amount of literature describing molecular cues governing vascular maturation, recreating vascular networks for therapeutic application, specific to a variety of tissue and organ sites, remains elusive.
Despite the fact that tumor development could be promoted in part by the immune system itself, the suppressive function of Treg-cells, as well as the molecular mechanism underlying their trafficking to tumor sites remains elusive.
Indeed, both acetylation and c-Abl-mediated phosphorylation have been correlated to WRN delocalization from nucleoli into DNA damage-induced foci [ 35, 36], but the mechanism underlying building-up of WRN at fork stalling sites remained elusive.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com