The metals were then eluded with 1 M HNO3 and analysed by Zeeman AA spectroscopy (Willie et al. 1998).
25(OH D was eluded with methanol/water (85:15, v/v) and the elute was monitored at 265 nm by a diode-array detector (UV6000; ThermoFinnigan, San Jose, CA, USA) with a 5-cm detector cuvette.
Afer heat treatment (60°C, 30 min), the resulting supernatants were loaded on to an immobilized metal affinity column (Novagen, USA), and eluded with 1 M imidazole, 0.5 M NaCl, and 20 mM Tris HCl buffer (pH 7.9).
The resulting supernatants were loaded onto an immobilized metal affinity column (Novagen, USA), and eluded with 0.4 M imidazole, 0.5 M NaCl, and 20 mM Tris-HCl buffer (pH 7.9).
The resulting supernatants were loaded on to an immobilized metal affinity column (Novagen, USA), and eluded with 1 M imidazole, 0.5 M NaCl, and 20 mM Tris HCl buffer (pH 7.9).
He was charged with eluding, possession with intent to distribute marijuana and other charges.
There, compatriots elude surveillance with e-mailed software and upload videos on dial-up connections.
Sykora tried to elude Cote with a move from right to left and Cote whacked Sykora in the midsection with his stick.
The home side increased the pressure but a finishing touch continued to elude them, with Dejan Lovren heading over and Philippe Coutinho firing against the post.
However, some B. anthracis strains can express functional but antigenically altered forms of PA, and such strains may elude treatment with such anti-PA antibodies.
