DOI: http://dx.doi.org/10.7554/eLife.01751.010 10.7554/eLiFigure51.011 Figure 3 figure supplement 4. Structural elucidation of TAGs by CID MS reveals fatty acid (FA) components with 2, 3, 5, 16, or 18 carbons in length and 0 2 double bonds.
DOI: http://dx.doi.org/10.7554/eLife.01751.009 10.7554/eLiFigure51.010 Figure 3 figure supplement 3. Structural elucidation of sex-specific TAGs using gas chromatography MS. GCMS analysis of the male-specific TAG fraction following trans-esterification confirms that tiglic acid is one of the fatty acyl components.
10.7554/eLife.01751.007 Figure 3. Structural elucidation of sex-specific TAGs.
Elucidation of the biochemical pathways underlying TAG synthesis is needed to better understand whether dietary sequestration or de novo production is preferentially used by desert-adapted drosophlids and to determine the ancestral state of TAG production.
Aiming at fundamental elucidation of the molecular mechanisms involved in N starvation-induced TAG accumulation, the global gene expression changes were identified by using suppression subtractive hybridization (SSH).
The MMTV-ErbB2, p53-null and C3(1)-SV40 tag mouse mammary gland cancer models are valuable tools for the elucidation of the mechanisms of mammary tumorigenesis [ 3].
We agree that synthesis of TAGs with variant substitution positions will be highly valuable for structural elucidation.
The availability of a large number of expressed sequence tags (ESTs) from C. trifoliata is also an excellent source of experimental material for elucidation of gene expression and regulation.
Elucidation of the underlying glycerol-assimilation mechanism will provide great potential in constructing an industrial strain with high TAG yield.
Taken together, these detailed data shed light on the elucidation of differential responses to nutrient stresses and may provide insights into future engineering of this promising alga for improving TAG and astaxanthin production.
We anticipate that the selectivity tag technique will be broadly applicable to nanopore-based protein detection, analysis, and separation technologies, and to the elucidation of protein translocation processes in normal cellular function and in disease.
