Using these two-dimensional methods, all of the structures could be elucidated unambiguously, and complete assignments of H and C signals were established (Table 1).
Systematic further study is needed to unambiguously elucidate the role of solvent for the hole filling by diluted PDMS, and why sub-100-nm holes are so difficult to fill.
The structures of -evodiakine were unambiguously elucidated by 1D and 2D NMR spectra, mass spectrometry, and single-crystal X-ray diffraction.
In view of the above mentioned facts and in continuation of our interest, the structure of (E -3-mesityl-1- naphthalen-2-yl) prop-2-E -3-mesityl-1- naphthalen-2-ylucidatE -3-mesityl-1- naphthalen-2-ylraction tE -3-mesityl-1- naphthalen-2-yl
The fatty acids could be unambiguously elucidated from the pattern of 1H COSY, 13C HSQC and 13C HMBC as 3′ hydroxyl lauroleic acid for the more hydrophilic fractions 60 61.
Unambiguously elucidating the absolute value and architecture of such complex repeat regions is not possible with other short fragment or hybridization-based methods.
It was demonstrated that both mass analyzers complement each other and that, even in the sub-nanogram range, the resulting set of MS data can be successfully used to elucidate most of the metabolic changes unambiguously, solely by mass spectrometric techniques.
We were unable to separate both compounds, however, succeeded to assign all NMR signals in the mixture and elucidated the structures of 2 and 3 unambiguously; the 1H 1H COSY spectrum was clearly resolved and indicated three spin systems for each component (see Fig. 2).
In particular, we paid special attention to the application of near-edge X-ray absorption fine structure (NEXAFS) to elucidate the chemical species and electronic structure and to unambiguously identify the level of unsaturation in the plasma-polymerized films.
Using the microfluidic chamber, we can unambiguously determine the nature of progeny virions targeted to axons and begin to elucidate the effectors of viral transport.
