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Mouse keratinocytes transfected with shRNA for SIRT1, p53 and Sp1 or treated with Sirtinol were also co-transfected with the murine core TERT promoter-Luc reporter vector pGL3-347-Luc pGL3-347-Luc pGL3-347-Lucing site along with the Renilla lucontaininglasmid as described elsewhere (MethedSp1
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Details about overall study conduct and reliability testing are described elsewhere; 17 26 methods related to the analyses presented are described below.
"But I don't see any reason to employ the same methods elsewhere".
Controls were included as advised elsewhere [45] (see methods).
We have published details of the study objectives, design, and methods elsewhere.
The generation of SAA1/SAA2 double knockout mice is described elsewhere in the Methods section.
The authors of the survey have provided a more detailed description of their methods elsewhere.
Sources of other reagents either are specified elsewhere in the methods or were previously reported (Rossi et al., 2009).
Discussion of FP with the spouse has been found to be critical in the use of FP methods elsewhere.
However, as documented elsewhere, [ 14] methods for identifying subgroup effects can be abused and results over-interpreted and measures should be taken to avoid this.
The TPC was assessed using Folin Ciocalteu reagent following reported method elsewhere [64].
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CEO of Professional Science Editing for Scientists @ prosciediting.com