Through the novel Single Donor vs. Elite Panel method we were able to identify within QTL confidence intervals SNP markers that would be suitable for marker-assisted selection of gray leaf spot resistant genotypes containing the above-mentioned GLS resistance QTL.
Method: Elite triathletes (n = 11) ran in time to self-selected motivational music, a neutral equivalent and a no-music control during submaximal and exhaustive treadmill running.
It can be expected that the target of 15 t/ha can be achieved by using R1128 in combination under the technical guidelines of "elite seeds, correct method, suitable field and better ecology" proposed by Yuan Longping.
Bound antibodies were detected by an avidin-biotin complex method (Elite ABC Kit, Vector) with 3,3'-diaminobenzidine as chromogenic substrate following the manufacturer's instructions.
The protocol for immunohistochemistry was based on the published ABC method (Elite ABC kit; Vector, CA, USA) using the rabbit anti-stathmin1 primary antibody at 1∶500.
Immunostaining was performed using an avidin-biotin complex peroxidase method (Elite PK-6200, Vector, Burlingame, CA, USA).
Immunohistochemistry of hTERT (clone 44F12; Novocastra, Newcastle upon Tyne, UK) (Yan et al, 2004) and γH2AX (clone JBW301, Millipore/Upstate, Mississauga, ON, Canada) was performed manually at dilutions of 1 : 25 and 1 : 1000, respectively, incubated overnight at 4°C and immunodetected using the Vector Elite avidin biotin complex method detection system (Vector Laboratories, Burlingame, CA, USA).
Fourthly, an elite stochastic learning (ELS) method is used to refine the solution.
Method: Two hundred and seventy elite junior Australian football players were screened using five hip and groin musculoskeletal tests.
