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A similar backward elimination screening algorithm as that used in MPAS is then applied using the scores defined above.
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The feminization alternative is eliminated in later steps of this process-of-elimination screen.
The process-of-elimination screen for SA-ZD has limitations.
To maintain consistency, subadult sex ratios were measured in the same way and at the same times in all three generations of the process-of-elimination screen.
No, because it is based exclusively on negative evidence from a process-of-elimination screen, rather than on positive evidence of mechanistic causation.
The process-of-elimination screen ruled out feminization and identified SA-ZD as a causal agent for the increased sex ratio bias in subadults compared with embryos.
I begin by describing a general process-of-elimination screen to disentangle X-linked SA-ZD from other potential factors leading to sex ratio distortion.
The process-of-elimination screen described here should provide the requisite tool to uncover SA-ZD when present alone or in combination with other sex ratio distorters, so long as it is sufficiently strong.
Each of these stocks had a sex ratio of approximately 85% females, and one of these (RIL-6) was used as the "SR" line in process-of-elimination screen for SA-ZD.
The SR line used in the process-of-elimination screen to test for SA-ZD was produced by crossing SKEW males to EVEN females and then making recombinant inbred lines (RILs) by repeated brother/sister matings for at least 15 generations before being used in process-of-elimination assay.
Because I passed through steps 1, 2a, 3a, and 3b, I can also conclude that the SA-ZD phenotype was operating and not confused with CYTO-ASEX, CYTO-FEM X-FEM, and Y-FEM, as described more fully in the last paragraph of the previous section describing the rationale for the process-of-elimination screen.
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CEO of Professional Science Editing for Scientists @ prosciediting.com