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A high throughput method for sequencing organisms from environmental samples would be facilitated by elimination of the sequence rearrangements.
A limitation for evaluation of specific Dido3 function in the Dido3ΔNT mouse mutant is that elimination of the sequence encoding the Dido gene N-terminus in the germline also affects Dido1 and Dido2 isoforms.
A particularly useful approach consists in flanking the DNA sequence to be deleted by tandemly oriented 34nucleotide-longnucleotide-long, which are recognised by Cre recombinase (Cre), a recombination enzyme that leads to the elimination of the sequence present between the two lox sites.
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Elimination of the DNA sequence that encodes the gene of interest is a reliable way to ensure complete elimination of the encoded protein's function.
It is tempting to speculate that some ciliate introns may evolve into IESs by providing a nucleus for the evolution of extended repeats, which then lead to the elimination of the enclosed sequence during macronuclear development.
Since exon 1 encodes part of the signal peptide for IL-7, elimination of this sequence should cause ECFP to accumulate inside producing cells and enhance visualization.
Minicircle vectors allow for sustained expression of a reporter as a result of the elimination of the bacterial sequences from the parental plasmid that interfere with expression.
This strategy allows for easier elimination of sequence deviations caused by sequencing errors in the sequenced or the reference genomes.
Authors have previously described them as merely the result of recombination between the proximal LTRs of two adjacent retroelements, leading to the elimination of the intervening genomic sequence.
Second, the pre-miRNA sequence placed in the 3'-UTR exerts an inhibitory effect on the protein gene expression, and this effect can be removed with the elimination of the pre-miRNA sequence by the intron splicing.
Firstly, we tested whether we correctly recover the previous mitochondrial sequences assembling from scratch in a pool of the total reads of the tagged library but after elimination of the species-specific sequence barcodes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com