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Alternatively, the same trick can be exploited to introduce an RNA interference based gene-silencing cassette, eliminating expression of a target gene in a subset of cells.
First, we showed that reducing or eliminating expression of MISC-1/OGC resulted in mitochondrial fragmentation in C. elegans and in human cells.
The first error term is excluded from analysis by eliminating expression values at or below background levels.
Here, we characterize and report the first tardbp mutation in zebrafish, which introduces a premature stop codon (Y220X), eliminating expression of the Tardbp protein.
The two main sources of heterogeneity in gene expression variations – additive and multiplicative components – are excluded in our analysis by eliminating expression values at or below background levels and by log-transformation of the data.
We reasoned that an alternative strategy would be to leverage tissue-specific silencer elements to delimit transgene expression to tissue types of interest by eliminating expression in non-targeted regions.
Similar(52)
Similar results were found in isolated anterior half-embryos: mutations of any of the Pitx-binding sites in E3 or E5 completely eliminated expression in the cVg1-expressing corner of the isolated half.
The gene for the predicted pantetheine binding protein is interrupted by an IS5 insertion and hence would not be expressed, and the insertion might also decrease or eliminate expression of the predicted oxononanoate synthase.
One approach to the production of these cells is gene editing to eliminate expression of the endogenous T cell receptor (TCR).
Genetic engineering of donor pigs to eliminate expression of the dominant xenogeneic antigen galactose α1,3 galactose (Gal) has created a sea change in the immunobiology of xenograft rejection.
Consistent with this hypothesis, it has been reported that independent petal losses within buttercup family (Ranunculaceae) were strongly associated with decreased or eliminated expression of a B-class gene, APETALA3-3 (AP3-3) (Zhang et al. 2013).
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