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The exhaustive digestion of heparin significantly affects the biological activity of the oligosaccharides produced by eliminating binding specificity [ 42, 43].
This negative control showed that DEAA quadruple mutations were in fact effective at eliminating binding for the targeted modes.
Addition of 0.01% Triton X-100 disrupted the colloidal interaction between Evans Blue and albumin and raised the apparent Kd by at least 500-fold, essentially eliminating binding.
We also generated serine 10 mutants in which three conserved amino acids within the BH3 domain of Puma were mutated (ΔLRR), thus eliminating binding to anti-apoptotic Bcl-2 proteins and rendering the protein non-apoptotic.
A recombinant protein rD-7 (here referred to as UspA1 527 665)) based on this region of UspA1 has been shown to block binding of multiple respiratory pathogens, virtually eliminating binding of Nm and Hi isolates to human lung epithelial cells and substantially reducing Mx interactions (Hill et al, 2005).
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He would eliminate binding arbitration for public employees and negotiate all union contracts from scratch.
Proteome data was specifically applied to guide the modification of a known IMAC binding protein, triosephosphate isomerase (tpiA gene product), and the use of site directed mutagenesis eliminated binding properties.
The GAVEL amendment guaranteed every bill a hearing, stripping Committee Chairs of their 'pocket veto,' eliminated binding, secret caucuses and more.
Klaffky, in a brief phone interview, said that as a lobbyist for StudentsFirst he did not work on some of the bill's toughest provisions, including language that limited collective bargaining rights among public employees (including teachers) for benefits, or language that eliminated binding arbitration and prohibited those same employees from striking.
Extremely minor chemical changes such as the addition of a single methyl group to a binding partner can sometimes suffice to nearly eliminate binding; for example, the aminoacyl tRNA synthetase specific to the amino acid valine discriminates against the very similar side chain of the amino acid isoleucine.
Further, mutation of basic amino acids 80 84 eliminated binding of EBER-1 and reduced, but did not completely eliminate, binding of 28S rRNA (Fig. 4).
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