Coinjection of a p53-targeted morpholino, which eliminates off-target effects caused by morpholino injection, had no observable effects on any of the gene expression patterns analysed.
Using this study design, they were able to show that quasi-randomization eliminates off-season effects of influenza vaccination, supporting the interpretation that study design and data analysis are crucial here.
Especially the high safety demands and the need to eliminate off target effects in humans require extensive research and development efforts.
Initially we tested SUM159 cells with two shRNA expression vectors (TβRIII-KD and TβRIII-KD4) to eliminate off target effects of the shRNA (Additional file 2: Figure S2).
One of the key elements in the peptide-TCR interaction is the HO-4 group of the galactosyl moiety, because the substitution of galactose by glucose, which only affects the inversion of the stereochemistry of hydroxy group at position C4, eliminated off T-cell activation.
El Paso said it would sell $2.25 billion in assets, cut capital spending, issue new stock and eliminate off-balance-sheet activities that have raised investors' eyebrows.
After watching this foolhardy but physically gifted and decent guy escape so many traps, we have a great deal invested in him emotionally, and yet he's eliminated, off-camera, by some unknown Mexicans.
These results demonstrate that engineered miR-124 responsiveness can eliminate off-target replication by unattenuated oHSV without compromising oncolytic activity, thereby providing increased safety.
This vector expression was specifically suppressed in hematopoietic lineages and successfully used to eliminate off-target expression of transgenes [13].
JEG-3 cells were transfected with either siLuciferase (siCon) for control or two different sets of GRP78 siRNA duplexes which were used to eliminate off-target effects.
An additional electronic gate was constructed about the diagonal region of a dot plot of pulse area vs pulse height for Hoechst fluorescence emission signals to eliminate off-diagonal events corresponding to doublets and higher order aggregates.
