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As a result of the genetic organisation of the virRS operon, the original insertional inactivation of virR in TS133 also eliminated the expression of virS [41].
The deletion of St6gal1 in mice eliminated the expression of the Sambucus nigra agglutinin (SNA) epitope [16], which is also recognized by SSA.
In contrast, BMP4 completely eliminated the expression of GalC in treated cells.
First, they either eliminated the expression of all gamma-protocadherins, or allowed the expression of only a single working isoform.
also showed that DOX withdrawal eliminated the expression of B-Raf within established adenocarcinomas, but those established tumors did not regress.
As expected, targeting the C-terminal end of ACOX2 both reduced the expression of the 75 kDa band, and eliminated the expression of the lower molecular weight bands.
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Short hairpin RNAs expressed by a lentiviral vector were used to eliminate the expression of all endogenous survivin isoforms, and an in vivo Bcl2 IP was performed on survivin-ΔEx3 positive- and negative-HeLa cell lysates.
TALENs were used to eliminate the expression of the endogenous TCR α and β chains to improve the expression and functionality of transgenic virus-specific TCRs (Berdien et al., 2014).
We find that FOXC2-KD in HMLE-Snail cells eliminates the expression of ZEB1, vimentin and fibronectin while it restores the expression of E-cadherin, thus inducing a complete MET irrespective of SNAIL overexpression (Fig. 6c).
One could have the genetic correlate of a trait and that trait could be the result of natural selection, and yet changing the environment could eliminate the expression of that trait.
This strategy was based on the principle that lowering (but not eliminating) the expression of a target gene using antisense technology would cause the bacteria to be more susceptible to an antimicrobial compound targeting the product of that gene.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com