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We then eliminated sequence redundancy with a sequence assembly program (CAP3).
MIC-limited DNA is found dispersed throughout the MIC genome at as many as 6,000 internally eliminated sequence (IES) sites [ 37].
Pre-processing of each of the 27 eukaryotes' largely single gene containing sequence files eliminated sequence redundancies that would introduce bias into the frequency calculations [ 39- 41], that would not be representative of the biological genomes.
Upon TSA treatment, IES (internal eliminated sequence) excision is defective [ 31] and incubation of cells with the C646 inhibitor at the beginning of sexual reproduction largely prevents the accumulation of H3K9ac/K14ac at the 5′-end of this stage (Additional file 3).
In order to cluster the 583 full-length LTR retrotransposons of oil palm into families based on sequence relatedness, we eliminated sequence redundancy according to the recommendations of Wicker et al. [ 1], i.e. elements are deemed related if a sequence identity of at least 80%% is detected across 80%% of the length of the retrotransposon.
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The ciliate Oxytricha trifallax engages in a genome-wide rearrangement process whereby its germline undergoes extensive removal of internal eliminated sequences (IESs) and unscrambling of macronuclear-destined sequences (MDSs).
The 13 eliminated sequences had an average AT content of 52.6 while the retained sequences had an AT content average of 68.8.
About 6,000 "internal eliminated sequences" (IESs) ranging from 0.5 to >20 kb in length, are removed, and the flanking macronucleus destined sequences (MDSs) are ligated.
Pairwise alignment was used to compare a selection of eliminated sequences with known MS4A protein sequences to verify that CDART accurately removed nonconserved proteins.
We also eliminated sequences with 8-TMs but no N-terminal signal peptide (8%) and proteins not showing characteristic serpentine receptor transmembrane topologies according to different prediction programs.
Chromosome breakage is accompanied by loss of a small amount (<75 bp) of DNA termed "breakage eliminated sequences" (BESs), coordinated with new telomere addition to form stable MAC chromosome ends.
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