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Those year industry combinations in which this requirement was not fulfilled were thus eliminated from the sample.
Plots deemed not to fall on closed canopy forest were eliminated from the sample by a 70%% canopy cover criterion.
Those who were unable to be assessed for BMI because of a missing height or weight measurement were eliminated from the sample, as were those who were missing data for either both grip tests, both gait tests, either of the exhaustion questions, either of the low physical activity questions, or either of the weight loss questions.
The representativeness of this random selection is shown in Figure 2. Participants with unconfirmed positive ELISA results were eliminated from the sample pool.
Only 3 subjects from the sample (0.04%) were receiving endocrinological treatment and they were not eliminated from the sample.
The prevalence of low serum folate (≤6.8 nmol/L) was eliminated from the sample of seniors and the proportion of elderly participants with low stores as indicated by RBC folate levels (< 373 nmol/L) was reduced from 2.5%to1.6%6%.
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Genomic DNA was eliminated from the samples by DNase treatment according to the manufacturer's description (DNA- free, Ambion, Austin, TX, USA).
Genomic DNA was eliminated from the samples by a DNase treatment (Fermentas) according to the manufacturer's description, and its concentration was again determined by spectrophotometry.
In all samples, the nitrogen contribution was at the limit of XPS resolution, suggesting that nitrogen species are quickly eliminated from the samples even at 200 °C in agreement with other reports.
Total RNA was isolated from the dissected tissues using the TRIZOL reagent (Invitrogen) method and genomic DNA was eliminated from the samples by RQ1 RNase-Free DNase (Promega) according to the manufacturer's instructions.
RNA was precipitated in 0.6 volumes of isopropylic alcohol and 10% sodium acetate 3 M pH 5.2 overnight, and further treated with the MicrobENRICH kit and Turbo DNase (Ambion, Tx) as prescribed by the manufacturer to ensure that contaminating eukaryotic RNA and eukaryotic and bacterial DNA were eliminated from the samples.
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