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With these additional steps, the interference was reduced, but not eliminated (data not shown).
RT-PCR experiments also confirmed that HDT1 and HDA6 mRNA levels were knocked down several fold in the T3 RNAi lines relative to non-transgenic plants, but were not eliminated (data not shown).
When the pH of each mineral leachate was adjusted to pH 7.0, using 1 M sodium hydroxide, bactericidal activity was eliminated (data not shown), demonstrating an important role for low pH conditions in antibacterial activity.
Unfortunately, abundant proteins were not significantly depleted, while less abundant proteins were reduced or sometimes eliminated (data not shown) [ 24].
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Finally, we found that laser cutting of the spindle, which relieves astral microtubule-dependent pulling forces, eliminated invaginations (data not shown).
We have made several Nox2/4 chimeras with slightly different junctions, and we find that small differences in position at which the DH domain begins can drastically decrease or eliminate activity (data not shown).
Eliminating excitatory synaptic transmission between RBCs and A17s with the AMPA-receptor antagonist NBQX eliminated the correlations (data not shown) and dim backgrounds reversibly reduced correlation strength (dim CCpeak = 0.34 ± 0.02, p = 0.0053, n = 8 pairs; Figure 2C E).
In control experiments, heat inactivation of ApHMW1C eliminated glycosylation activity (data not shown).
Gel shift experiments with recombinant dTCF demonstrated that the introduced mutations eliminated dTCF binding (data not shown).
The effect of these "body fatness" SNPs on UAC, WHR and two skin fold measures (TrS and SbS) appeared to be mediated through adiposity, since adjusting for PC1 (as a surrogate measure for "body fatness") completely eliminated the associations (data not shown).
Addition of a blocking peptide specific for the epitope targeted by our antibody eliminated all staining (data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com