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Although we did not analyze the exact nature of the sequences removed, it was comforting to see that the fraction of eliminated cells was close to the actual incidence of amber stop codon-containing clones in the different libraries.
A third possibility is that defective ploidy could be a defect common to the eliminated cells.
As a further difference, the eliminated cells due to competition are not replaced by cell proliferation.
If not eliminated, cells having DNA damage could potentially become transformed by further mitogenic stimulation and DNA mutations [ 22, 23].
If BIR is eliminated, cells lacking telomerase senesce and undergo a complete loss in viability (Lydeard et al, 2007).
Similar to the IGROV-1 cells, G-1 treatment also significantly decreased SKOV-3 ovarian cancer cells in the G1 phase, increased cells in the prophase, and eliminated cells in metaphase, anaphase, telophase, and cells undergoing cytokinesis.
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The molded funnel-shaped inlet eliminated cell clogging that we observed in conventional cylindrical shaped inlets formed with hole punchers.
In this way, we eliminated cell lineage-specific biases and were able to detect tumor-specific DNA methylation changes.
Therefore, we sought to develop whole cell assays of Rac1 activity during cell-cell adhesion under conditions that eliminated cell spreading and integrin-based adhesion.
Next, medium was collected, centrifuged to eliminated cell debris, and stored at −20°C until use.
Studies have shown that the gene works by revving up production of killer cells that directly target and eliminate cells infected with HIV.
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