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Short hairpin RNAs expressed by a lentiviral vector were used to eliminate the expression of all endogenous survivin isoforms, and an in vivo Bcl2 IP was performed on survivin-ΔEx3 positive- and negative-HeLa cell lysates.
TALENs were used to eliminate the expression of the endogenous TCR α and β chains to improve the expression and functionality of transgenic virus-specific TCRs (Berdien et al., 2014).
One could have the genetic correlate of a trait and that trait could be the result of natural selection, and yet changing the environment could eliminate the expression of that trait.
Subsequently, DN1Tg mice were bred onto hCD1Tg/Rag-/ background to eliminate the expression of endogenous TCR.
It will be critical to also eliminate the expression of the mutant form of the protein.
This would eliminate the expression of endogenous TCR that may interfere with the analysis of DN1 T cells.
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We find that FOXC2-KD in HMLE-Snail cells eliminates the expression of ZEB1, vimentin and fibronectin while it restores the expression of E-cadherin, thus inducing a complete MET irrespective of SNAIL overexpression (Fig. 6c).
As a result of the genetic organisation of the virRS operon, the original insertional inactivation of virR in TS133 also eliminated the expression of virS [41].
The deletion of St6gal1 in mice eliminated the expression of the Sambucus nigra agglutinin (SNA) epitope [16], which is also recognized by SSA.
This strategy was based on the principle that lowering (but not eliminating) the expression of a target gene using antisense technology would cause the bacteria to be more susceptible to an antimicrobial compound targeting the product of that gene.
Eliminating the expression of the male form of the neural sex determination factor, Fruitless (FruM), in three OA suboesophageal ganglia (SOG) neurons also leads to increased male-male courtship behavior in these same contexts.
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