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He would eliminate binding arbitration for public employees and negotiate all union contracts from scratch.
Extremely minor chemical changes such as the addition of a single methyl group to a binding partner can sometimes suffice to nearly eliminate binding; for example, the aminoacyl tRNA synthetase specific to the amino acid valine discriminates against the very similar side chain of the amino acid isoleucine.
Further, mutation of basic amino acids 80 84 eliminated binding of EBER-1 and reduced, but did not completely eliminate, binding of 28S rRNA (Fig. 4).
Mutation of either BRK site reduced (slightly) but did not eliminate binding while mutation of both sites resulted in no detectable binding.
Interestingly, although the point mutation in STAT5 binding site was shown to completely eliminate binding activity in Ba/F3 murine pro-B cells [12], it revealed only a subtle decrease in the Bcl-X promoter activity as shown here.
Treatment of the TDP-43/TIA-1 complex with RNase eliminated binding of endogenous TDP-43, but did not eliminate binding of over-expressed TDP-43, which suggests that binding can occur, but is concentration dependent.
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Proteome data was specifically applied to guide the modification of a known IMAC binding protein, triosephosphate isomerase (tpiA gene product), and the use of site directed mutagenesis eliminated binding properties.
The GAVEL amendment guaranteed every bill a hearing, stripping Committee Chairs of their 'pocket veto,' eliminated binding, secret caucuses and more.
Klaffky, in a brief phone interview, said that as a lobbyist for StudentsFirst he did not work on some of the bill's toughest provisions, including language that limited collective bargaining rights among public employees (including teachers) for benefits, or language that eliminated binding arbitration and prohibited those same employees from striking.
Intriguingly the Glu379 mutant, which had no effect on syntaxin binding (Figure 1C), completely eliminated binding to Mint1 (Figure 1D).
Furthermore, as expected, mutation of basic residues 80 93 eliminated binding of L22 to EBER-1 (Fig. 4A, lower panels).
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