Growth pattern under elicitation was determined, where cultures under pectin and MnCl2 reported early cell death which was due to the deleterious effect of elicitation.
This might be due to the excessive consumption of the enzyme in combating the increased ROS level under elicitation.
The change in the activity of SOD under elicitation was too prominent; therefore, it can be said that its presence in all samples suggests that this enzyme may participate in protecting suspension cells against free superoxide radicals.
Resveratrol accumulation is strictly controlled at the transcriptional level by regulation of the steady state of stilbene synthase transcripts [ 54], both during development [ 23] and under elicitation [ 24- 27].
The results of this study are also in accordance with the previous study that the higher enzyme activity and antioxidant properties are attributed to increased TQ and THY production in them under elicitation which are known antioxidants.
Likewise, in the case of cultures grown under elicitation of pectin 15 mg/L and MnCl2 10 mg/L, highest zone of inhibition was recorded against B. cereus (30 ± 0.7) and E. coli (30 ± 0.2) (Table 5) with MIC values 2.55 ± 0.6 and 2.46 ± 0.2 µg mL−1 (Table 4), respectively, at lowest dose of 25 µL (25 mg sample).
Next, further consideration revealed that under the elicitation function, three more subordinate functions were necessary (elicitation, facilitation and clarification requests), while under the feedback function, another three subordinate functions were included (recast, comment and assessment).
The presence of these autoreactive costs could explain why some studies of the costs of immune elicitation only uncover effects under stressful conditions (e.g. Ilmonen et al. 2000; Moret and Schmid-Hempel 2000).
The TLC results under MnCl2 elicitation reported the production of THY along with four other metabolites with R f values 0.76, 0.97, 0.73, 0.60, and 0.43, respectively (Table 2).
The inhibition activity recorded for the cultures under pectin elicitation increased with the increasing elicitor concentration and pectin 15 mg/L elicitation recorded maximum inhibition comparable to the cultures without elicitation (control suspension), epicotyl explant extract, and to some extent the standard, that is, ascorbic acid over the 18.62 IC50 value (Table 3).
