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Although DCE can elicit current preferences in this context, using ex ante DCE to estimate the welfare costs of such a long-term intervention requires caution.
Cells were clamped to a 40 mV holding potential with voltage ramps (500 ms) from 60 mV down to −100 mV used to elicit current.
Although we were not able to elicit current from LLZ, we found that CHEB42A increased the current amplitude from another DEG/ENaC channel, ASIC1a, for which the ligand is known [14], [40].
Voltage steps were applied to elicit current and fluorescence signals.
A time frame of the past few days was chosen to elicit current symptom and mood states.
The set of child sessions involve meetings with the children to cover some of the same ground as that accomplished with the parents: orient them to the program, collect assessment data, elicit current concerns and wishes, begin developmentally appropriate psychoeducation, and elicit personal narratives.
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Once inhibition developed, increasing citral concentration did not elicit currents.
In fact, formaldehyde (1∼10 mM) did not elicit currents in ASIC1a-transfected CHO cells (data not shown).
A recent report has indicated that formaldehyde (>0.013 mM) can elicit currents via transient receptor potential vanilloid receptor 1 (TRPV1) and this current could be blocked by the specific TRPV1 antagonist capsazepine in dorsal root ganglion (DRG) neurons [8].
Rapid, local application of acetylcholine can elicit currents of similar amplitudes to those obtained with bath application in layer VI pyramidal neurons [9] but, in fact, often elicits smaller currents since the placement of the applicator may preclude the stimulation of nicotinic receptors away from the soma.
At a hyperpolarized holding potential of −84 mV a considerably larger driver voltage to the fluid jet, and hence force on the hair bundle, was needed to elicit currents from the mutant v2J/v2J OHC than was required for the control +/v2J OHC (Fig. 7).
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