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The development of recombinant antibody technology provides an alternative source of recognition elements with significant potential to satisfy the shortcomings of conventional antibody reagents.
The ~95 Mb Trichoplax genome sequence was compared with the Repbase database 14.04 [ 9] using tblastx [ 10] with an e-value threshold of 10-4, revealing 139 putative elements with significant similarity to known TEs (available in additional file 2).
The transcription of non-coding RNAs from genomic regions acting as enhancers has already been shown to occur in elements with significant sequence conservation, although little is known about the mechanism involved.
In particular, SECIS elements with significant scores were identified downstream of the TGA codon of DUF466 proteins of Helicobacter hepaticus (T = 19.6; P = 8 × 10−), Helicobacter cinaedi (T = 19.8; P = 7 × 10−), and C. lari (T = 11.1; P = 9 × 10−).
Hierarchical clustering of Pol II binding for LTR elements with significant enrichment in at least one cell line revealed that normal cell-lines clustered separately from cancer and transformed cells (Additional file 1: Figure S14).
Finally, repeat elements with significant sequence similarity to elements identified from well-annotated genomes were identified using RepeatMasker, with RepBase (version 16.04) (http://www.girinst.org/ [date last accessed 26 Sep 2011]) as a reference library.
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This vaulting in a floor system leads to a lightweight structural element, with significant weight savings compared to traditional concrete floor slabs.
Promoter elements with highly significant scores (see Materials and Methods) were detected for 1222 unique 3' cDNA ends, associated to 906 different genes, 202 TSSs had both −35 and −10 recognition elements for E-σ70; 475 had the −10 element only, and 424 with −35 element only.
Although they are the most abundant, Ogre elements are probably not the only Ty3/gypsy elements with a significant impact on pea genome evolution.
WAF-treated embryos had a general size reduction in all cartilage elements with a significant lack of anterior extension of the most prominent jaw elements (Meckel's and ceratohyal cartilage).
To identify repetitive element subfamilies with significant differences in tumor versus control we built a paired GLM in EdgeR using individual as a covariate.
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