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The poached elements were spotted in a private European collection by the French fossil trader François Escuillé, who notified Belgian palaeontologist Pascal Godefroit about them in 2011.
All elements were spotted in duplicates on UltraGAPS slides from Corning with an SDDC-2 ChipWriter (Biorad) yielding a total of 55.488 spots on each microarray.
The control elements were spotted 14 times each on the array.
These elements were spotted onto the microarray at a concentration of 200 pg/spot.
Elements were spotted on Corning UltraGAPS slides using a BioRobotics MicroGrid II TAS using MicroSpot 2500 pins.
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First, the floppy-brimmed headpiece, a distant descendant of the hats worn by fishermen to ward off the elements, was spotted on the heads of performers like Usher, Kid Cudi and Schoolboy Q at South by Southwest, the annual music jamboree in Austin, Tex.
It was first spotted online in March 2010, though elements of it were spotted by computer virus experts in summer 2009.
On the morning of 27 March, major elements of the Italian Fleet were spotted en route to the sea lanes between Egypt and Greece and the carrier sailed later that afternoon to intercept them.
The slides chosen for this study were 22,000 element Compugen array chips which were spotted with single stranded oligonucleotides of 60 base pair length.
All clones were spotted in triplicate, resulting in 97,299 elements over two sides [ 29- 31].
All clones were spotted in triplicate, resulting in 97,299 elements over two sides.
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