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The different elements were checked by the appropriate health-care professionals, who signed the corresponding section after completing the verification process.
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The reproducibility (± 5% for most elements) was checked by digesting one sample in triplicate.
Double mutants were confirmed by non-complementation with caps and trn alleles and the exact deletion sites induced by the P-element were checked by inverse PCR and sequencing.
To avoid repetitive elements in the genome, the primers were checked by in-silico PCR [ 65] and the amplicon sequences aligned to the whole genome assembly by BLASTn.
Analytical results were checked by using the finite element analysis.
568 * Elements are checked in key order.
396 * Elements are checked in key order.
Polymer compositions were checked by 1H n.m.r.
Colonies were checked by PCR.
Thermal stabilities were checked by thermogravimetric analysis (t.g.a).
Thermal stabilities were checked by t.g.a. and d.t.g.
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