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There she continued her investigations into telomerase, characterizing genetic elements of the enzyme and further elucidating its cellular functions.
We have uncovered many aspects of NO function including key elements of the enzyme nitric oxide synthase and the receptor for NO, the soluble isoform of guanylate cyclase (sGC).
Activation requires rearrangements to key structural elements of the enzyme's active site, which accompany cyclin binding and phosphorylation.
Molecular modeling, based on the OXA-1 crystal structure, suggested interaction of the aryl groups with the hydrophobic elements of the enzyme's active site and polar interaction of the keto and phosphonate groups with the active site residues Ser 115, Lys 212 and Thr 213 and with the non-conserved Ser 258.
QSAR correlations may bring information on both the structural elements of the enzyme and the structural elements of the substrate that together govern recognition and regioselectivity in a crosstalk that remains to be understood.
The critical component in this structural rearrangement is the trigger loop, a flexible element of the largest subunit of RNA polymerase (the β' subunit in eubacterial RNAP, and the Rpb1 subunit in eukaryotic RNA polymerase II (Pol II)) that interacts with the substrate and other elements of the enzyme active site.
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The formation of these hydrogen bonds facilitates the electron transfer from the B-ring of a bioflavonoid molecule to the hematin element of the enzymes.
Solubilisation of the human, membrane-bound P450 2E1 (2E1) was achieved by fusing key elements of the 2E1 enzyme with selected parts of P450 BM3.
The ensuing analysis of conservation at the level of QSOX domains was conducted to shed more light on the evolution of fundamental structural and mechanistic elements of the QSOX enzyme.
Hydrostatic high-pressure technology (HHP), which can effectively disturb or even completely destroy eukaryotic cell membranes, elements of the cytoskeleton, and enzyme systems [ 36– 38], allows for the inactivation of cholesteatoma cells on the ossicles.
The TR molecule, complementary to the telomeric repeats, is an important element of the telomerase enzyme; it consists of an RNA template region that facilitates adding telomeric repeats through the action of the reverse transcriptase catalytic subunit of telomerase (TERT) [ 10].
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