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Teacher M27, whose cluster also showed high coherence between CK and PCK elements (not shown), said: 'I am a better teacher now.
Residues that have methionines in several other species and are predicted to be part of secondary-structure elements (not shown) were chosen as sites for mutations.
Finally, whereas N-Myc deficiency alone decreased the size of the epiphyseal head of the tibia (and other limb elements – not shown), and resulted in small declines in expression of Ihh, Runx2, Vegfa in the growth plate, it had a relatively strong effect on Col1 and Runx2 expression in the perichondrium, and on the thickness of perichondrium (Fig. 9IB, B', F, 9IIF).
Dumbbell elements (not shown) are located 3′ to these SLs.
Cloning analysis revealed that the CsF3′5′H gene promoter contains several light-responsive promoter elements (not shown), further indicating that light might be a key factor in the control of CsF3′5′H transcription.
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The knockout of Sp_hsp104+ had no effect on the maintenance of [cif], indicating that Sp_Hsp104 is not involved in the propagation of this epigenetic element (not shown).
The PDH references the DBSequence element (not shown), which captures the native accession of the protein in the source database and, optionally, the protein sequence itself, description line and other basic details that can be extracted from the source database (such as a fasta file).
For example GA-responsive elements, but also water-stress and light regulated elements (data not shown) were found in all of them.
In particular, although lacking TSDs, it seems that the EgRep element previously described for E. granulosus by Marin et al. (1993) consists of a solo-LTR from a ta-TRIM element, embedded within a larger, sparsely repeated sequence lacking any other distinctive elements (data not shown).
(Results for these major elements are not shown here. However, as the same is true for the majority of the elements, the data for Zn reproduced in Fig. 5b serve to exemplify the point).
ChIP analysis with an anti-panRAR antibody revealed RAR binding to all elements tested (Fig. 5), whereas no RAR binding was observed to control sequences lacking DR2 elements (data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com